LOCUS       40924_23734       12334 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB717 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12334)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12334)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2921..2950
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2957..2986
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3002..3031
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3038..3067
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      3098..3350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3384..3400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3408..3424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3432..3462)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3477..3498)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3534..3786)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3824..5119)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61208.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3824..5119)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5171..5350)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5856..5880)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6401..7189
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7438..8026
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8212..8352)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8696..8890)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8959..10029)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10461..11087)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgggtt aattaacggt gaacaaaagc taatctccga
     2941 ggaagacttg aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gactcgacgg
     3001 tgaacaaaag ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga
     3061 agacttgaac ggtagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata
     3121 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     3181 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     3241 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     3301 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt
     3361 tccggaacca attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3421 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3481 gtgagctaac tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag
     3541 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
     3601 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
     3661 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
     3721 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
     3781 acgatcgggg aaattcgcct cgagtgactt ctctgattgc agactattta taccaaccag
     3841 caagaaagtg tcgaagtatg aagcagaagg cgcaggctag agcctggaaa acgagaagat
     3901 ggatgcaaag agacttttct gatttaggac caaacaacct caagtaaaca ttgacgagtg
     3961 tcccatcttt tgtgccggtc agtaatccgg tagcaggatc ataccttggg agacgatggc
     4021 gcggacattt cacaatccca gcaagctgag gaagcgataa tagaaggttc agaacttgag
     4081 gaagaaagaa gatgaggagg gtttcactga agtgacccag aataccaaca actgccatag
     4141 tcattccggc gaagactgtg taagtgtctc caacaaaaac tgaagaagga taccaattgt
     4201 aagcaagcat tgccaaggat gtggccatca gcggttgagt aagaaatatc gagaaggcat
     4261 gagcttggtg atactcggga tcaacagatg ctccgatttg cataacgttg tgtatcagga
     4321 tagcagcggc aataacaact gtttgaccga tttcaaggcc attcagacca gcgtggatgt
     4381 taatagagtt tgtacaaaag acagcaagta gccccatata taacttatat atccttccta
     4441 gattaagcac ttccaagcca atataagcaa caagaggttt tggtataaca atagttgtat
     4501 gaccagcata agccatcaaa agtggaaggg tagcaaaaga tggcaggaca agttttactc
     4561 tccaaggcac atcaaggaca tcatctacaa atccaagcaa aatcatgaag caaatagatg
     4621 ctagtgctgc attatactcc acaagccaat tagaatcttc agtgaagtta aaatactgaa
     4681 atattatcgc cacgatcaaa aagacaatac caacaacaat acccaatgac tctggcactt
     4741 taatatctcc ttgaggcgtg cctcttttgt tgatatcaaa accaaacatg ttacgtctca
     4801 gaacgtatct ggcagtaaca ggaatcattt tgagagtgac gaaaaaccca accaagctaa
     4861 gccccgcgtt aattagaatc gatcgcttga gttcattatc aaccttgtag tgaaaacaga
     4921 gaaggtatag atagagcgaa cagagaagag tagagatcac gaagatcact cctaatttcg
     4981 gcggcgcgag acgaaattcc tcaccggaag cagaaaccgt ctttcgggtc atcttctgct
     5041 ccgatggcgc tgagttgggt tccgacgggt ccggtttgtt gggaattgag atcgatgaag
     5101 ctctcttacg agctgccata tcttattgcc ccccgggatc agatctggat tgagagtgaa
     5161 tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac
     5221 aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt
     5281 ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac
     5341 gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca
     5401 taacgtgact cccttaattc tccgctcatg atcttgatcc cctgcgccat cagatccttg
     5461 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
     5521 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
     5581 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
     5641 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
     5701 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gctgatccgt
     5761 cgagattaat agaaataaat tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc
     5821 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     5881 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     5941 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     6001 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     6061 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat
     6121 tcgggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     6181 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     6241 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     6301 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     6361 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc
     6421 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     6481 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     6541 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     6601 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     6661 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     6721 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     6781 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     6841 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     6901 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     6961 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     7021 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     7081 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     7141 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc
     7201 tagaaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca
     7261 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     7321 gcgtgcataa taagccctac acaaattggg agatatatca tgcatgacca aaatccctta
     7381 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7561 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     7621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8041 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8101 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8161 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     8221 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8281 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     8341 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     8401 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     8461 ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc
     8521 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     8581 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     8641 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     8701 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     8761 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     8821 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     8881 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     8941 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     9001 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     9061 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     9121 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     9181 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     9241 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     9301 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     9361 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     9421 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     9481 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     9541 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     9601 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     9661 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     9721 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     9781 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     9841 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     9901 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     9961 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
    10021 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca
    10081 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
    10141 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
    10201 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
    10261 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
    10321 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
    10381 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
    10441 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
    10501 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
    10561 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
    10621 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
    10681 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
    10741 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
    10801 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
    10861 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
    10921 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
    10981 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
    11041 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
    11101 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
    11161 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
    11221 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
    11281 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
    11341 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
    11401 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
    11461 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
    11521 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
    11581 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
    11641 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
    11701 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
    11761 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
    11821 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
    11881 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
    11941 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
    12001 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
    12061 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
    12121 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
    12181 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
    12241 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
    12301 gcatgcacat acaaatggac gaacggataa acct
//
